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Scanning Probe Microscope based Tools for NanoTechnology
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Application Notes

Science & Technology Applications: Life Sciences (Biology, Biotechnology): Blood cells study.: Error Mode

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Blood cells study.

Blood smear

Suspension

Error Mode

Preparation and scanning artifacts

Virus-cell Interaction



Error Mode

Error mode is intended for examination of fine features of a surface with too different heights. We use the error mode for detection of 10-20 nm Z features on a sharp slope of an erythrocyte (height difference of 1 mkm).

a) 

b)

Fig. 1 The surface of a chicken erythrocyte with adsorbed influenza virus particles:
a – intermittent contact mode, three-dimensional view; b – error mode.

a) In the first case, small surface details (influenza virions) are either indistinguishable or visible only on the marked portion of the figure.
b) In the error mode, virions are observed on the whole surfaces of erythrocytes. Besides, artifacts caused by the probe excitation at sharp boundaries during scanning are clearly seen.

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