NTEGRA platform: NTEGRA Spectra
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Confocal microscopy
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| Optical module |
Inverted or upright microscope direct viewing system |
| Housing with VIS optics (390-800nm) |
| Polarizer in illuminator channel with Glan-Taylor prism 390-1000nm – manual |
| Polarizer in detection channel with Glan-Taylor prism 390-1000nm – motorized |
| 1/2 wave plate, motorized – 3 position |
| Beam splitter |
| Evanescent excitation option (for TERS) |
| Optical resolution |
XY |
200nm |
| Z |
500nm |
| Scanning module |
Sample weight |
Up to 1000g |
| Scanning range |
100x100x25 um |
| Closed-loop operation |
Capacitive sensors for 3 axes |
| Non-linearity, XY |
0.03 % (typically) |
| Noise level, Z |
<0.2 nm (typically) |
| Noise level, XY |
<0.5 nm (typically) |
| Pinhole |
Variable from 0 to 1,5 mm, step size 0,5 um |
Note that the sample for confocal microscopy can be either transparent or not and can be observed in air as well as in liquid environment.
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Spectroscopy
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| Spectrometer focal length |
520 mm |
| Laser wavelength* |
441, 488, 514, 532, 633 nm |
| Stray light rejection |
10-5 measured at 20 nm from 632 laser line |
| Flat field |
28 mm x 10 mm |
| Spectral resolution |
0.025 nm (1200 l/mm grating**) |
| Ports |
1 input, 2 output |
| Grating mounts |
4-position turret (3 gratings+mirror for "direct imaging" mode) |
| Detectors |
CCD |
Spectral response 200–1000 nm, thermoelectric cooling down to –80°C, 95 % quantum efficiency at 500 nm |
| Avalanche photodiode for photon counting*** |
Spectral response 400–1000 nm, dark counts = 25 counts/sec, supplied with PCI board with 1 GHz counting speed |
* Basic configuration includes 488 nm laser, additional lasers can be supplied optionally
** Additional gratings can be supplied optionally. Echelette gratings are available for the highest spectral resolution.
***PMT can be installed instead of APD
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Scanning Near-Field Microscopy
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| Shear Force Microscopy / SNOM reflection, transmission, luminescence(optinal) |
| Laser module |
Coupling unit |
X-Y-Z positioner, positioning accuracy 1 um |
| V-groove fiber holder |
| Coupling 40x objective |
| Fiber Delivery System KineFlex |
| Beam attenuator |
Variable neutral density filter |
| Shear Force Imaging |
Sample size |
Up to diameter 100mm, up to 15 mm in height |
| XY sample positioning range |
5x5 mm |
| Sample positioning resolution |
5 um |
| Closed-loop operation |
Capacitive sensors for 3 axes |
| |
Scanning by sample |
Scanning by probe |
| Scan range |
100x100x25 um |
100x100x7 um |
| Non-linearity, XY |
0.03 % (typically) |
<0.15% |
| Noise level, Z |
<0.2 nm (typically) |
0.04 nm (typically),<=0.06 nm |
| Noise level, XY |
<0.5 nm (typically) |
0.2 nm (typically), <=0.3 nm |
| Quartz tuning fork base frequency |
190 kHz |
| Aperture diameter |
<100nm |
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Channels for simultaneous registration
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Reflection |
| Transmission/Fluorescence |
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PMT detectors
for each channel
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Spectral response |
185-850 nm |
| Sensitivity at 420 nm |
3x1010 |
| Vibration isolation |
Dinamic |
0.7-1000 Hz |
| Passive |
above 1 kHz |
| Scanning probe microscopy |
| AFM (contact+semi-contact+non-contact)/Lateral Force Microscopy/Phase Imaging/Force Modulation/Adhesion Force Imaging/Magnetic Force Microscopy/Electrostatic Force Microscopy/Scanning Capacitance Microscopy/Kelvin Probe Microscopy/Spreading Resistance Imaging/Lithography: AFM (Force and Current) |
| Sample size* |
Up to diameter 100mm, up to 15 mm in height |
| Scan range |
50x50x5 um |
| Closed-loop operation** |
Capacitive sensors for 3 axes |
| Non-linearity, XY |
<0.15% |
| Noise level, Z |
0.06 nm (typically), <=0.07nm |
| Noise level, XY |
0.1 nm (typically), <=0.2 nm (XY 50 um) |
*Scanning head can be configured to serve as a stand-alone device for specimens of untimited sizes
**Built-in capacitive sensors have extremely low noise and area down to 50x50 nm can be scanned with closed-loop control.